Immunohistochemical Characterization of Frog Rana ridibunda Chromaffin cells Using a Monoclonal Antibody Against Dopamine
*Dr Mohamad Khouder MORRA
College of Education
Sciences department
SALALAH
Sultanate of Oman
Abstract
The adrenal gland of amphibians is composed of a mixed population of adrenochromaffin and corticosteroid-secreting cells.
To localize chromaffin cells, previous immunohistochemical studies conducted on adrenal gland have used antisera against the enzymes implicated in the biosynthesis of cathecolamines such as anti-tyrosine hydroxylase, anti-phenylethanolamine-N-metyltransferase, or dopamine-B-hydroxylase.
In the present study, we demonstrate by immunofluorescence procedure the localization of frog adrenal chromaffin cells using a monoclonal antibody directed against dopamine substance . In addition, our immunohistochemical study confirms the co- localization of dopamine and adrenaline in adrenal chromaffin cells.
